Are you using SDR Console and have you tested you set up with other software?
Because I also had a similar problem with my Pluto some time ago. No output power - but everything worked the day before. The setup was not changed in the meantime.
First I was worried that my Pluto was damaged. But it turned out that due to a Windows update my USB headset stopped working properly. It seems that if there's no properly working microphone present in the SDR Console no power gets out of the Pluto - even if you tune or do a tone test.